The yeast expression system is widely used in industrial production. In addition to the clear and safe genetic background, yeast expression system harbors the advantages of both prokaryotic and eukaryotic expression systems: fast proliferation, high-density fermentation in economic media, supporting complex post-translation modification, and strong secretion expression ability, to effectively reduce downstream purification costs. Nanobody can be encoded by a single gene and expressed in large amounts in microorganisms such as yeast through genetic engineering due to its small molecular weight and simple structure.
Yaohaibio provides high-expressed strain nanobody selection and fermentation process development services. With high-densitied fermentation, over 10 different types of nanobodies are expressed by Pichia pastoris PAOX1 induction or PGAP, and the expression amount can be up to 10 g/L or more.