Circular RNA (CircRNA) is a type of non-coding RNA molecule without a 5' cap and a 3' poly (A) tail, forming a cyclic structure by a covalent bond. It is mainly located in the cytoplasm or stored in exosomes. Not affected by exonuclease, it is much stably expressed and hardly degraded. CircRNA is mostly formed by exon cyclization, some CircRNA lasso structures are formed by intron cyclization. According to the source, CircRNA can be roughly divided into 4 categories: ECircRNA with only exons, ElcircRNA with a combination of introns and exons, lasso CircRNA composed of introns, and CircRNA formed by intergenomic cyclization. It is currently a hotspot molecule in life science and medicine research.
Recent studies have shown that CircRNA is abundant in microRNA (miRNA) binding sites. It functions as miRNA sponge intracellularly, thereby eliminating the inhibition of miRNA on its target gene and raising the expression level of the target gene, which is called competitive endogenous RNA (ceRNA) mechanism. CircRNA plays an important role in regulating diseases through interactions with disease-associated miRNA.
CircRNA has obvious advantages as a biomarker for novel clinical diseases, and is of great research value due to its tissue specificity, disease specificity, timing specificity, and high stability.
CircRNA plays an essential biological role. Endonuclearly, it mainly affects the transcription of its parent gene and regulates the splicing of its linear homologous transcripts. Extranuclearly, it not only acts as a miRNA sponge, but also functions via binding proteins. Recent research has shown that CircRNA can be translated to functional polypeptides/proteins.
As a global CDMO service provider focusing on microbial expression systems, Yaohabio has established a mature CRO R&D service platform for innovative CircRNA therapies on the basis of a powerful R&D technology platform, mature quality analysis and control systems, and pioneering technology in cyclic RNA synthesis. The platform includes four technical modules: RNADes (CircRNA structure design and optimization platform) , RNASyn (CircRNA synthesis and modification platform), RNAPur (CircRNA purification platform), and RNAQua (CircRNA quality analysis and control). A complete technical platform enables Yaohaibio to realize efficient preparation and purification of CircRNA, and provide full-process, high-qualified CircRNA preparation CRO services in vitro for universities and scientific research institutions.
Cell evaluation: efficient and stable EGFP expression in CircRNA prepared in vitro, and strong fluorescence signals enabling being detected one week after cell transfection.
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