CRO Services for Novel Circular RNA Therapies
Officially launched

In the recent two years, with the successful development of new mRNA COVID-19 vaccines and the approval of several new RNA-based drugs, RNA has jumped to the forefront of drug research as an important role in conveying life information in the central law of biology.
Among them, mRNA technology has shown strong therapeutic strength in the COVID-19 epidemic, but it is imperfect with restricted ceiling, as there are many drawbacks such as instability.
Compared with linear RNAs like mRNA, circular RNAs have incomparable advantages such as higher stability, higher and longer maintenance of antigenic levels, and relatively simple production processes, which make circular RNA a promising solution to the technical challenges faced by linear mRNA.

Overview of circRNA
Circular RNA (Circular RNA, CircRNA) is a type of non-coding RNA molecules without a 5' end cap and a 3' terminal poly (A) tail, with a covalent bond to form a ring structure. It is mainly located in the cytoplasm or stored in exosomes. It is not affected by exonuclease, and is more stable in expression and not easy to degrade.
CircRNA is tissue-specific, disease-specific, and time-series-specific, as well as structurally stable and evolutionarily conserved. Studies have shown that circRNA plays important biological functions both inside and outside the nucleus. Within the nucleus, it mainly affects the transcription of its parent gene and regulates the splicing of its linear homologous transcripts; in the cytoplasm, it not only acts as a miRNA sponge, but also functions through binding proteins. Researches in recent years have shown that circRNA can translate functional polypeptide/proteins.

Yaohaibio Launches CRO Services for Circular RNA Innovative Therapies
As a domestic leader in CDMO service focusing on microbial expression system, Yaohaibio always insists on innovation and development, constantly improving innovative R&D and platform construction capabilities, and enhancing system construction of production, quality management and control services. Based on our complete technical advantages, we continue to deepen our technical researches in the field of RNA. In February 2022, Yaohaibio launched a diversified and integrated research-grade mRNA sample preparation service platform to promote the development of industrialization process in the industry.
Our innovation never stops. Based on the strong R&D technology platform, mature quality analysis and control system, and pioneering technology in circular RNA synthesis, Yaohaibio has established a mature CRO R&D service platform for circular RNA innovative therapeutics, which contains four major technology modules: RNADes (CircRNA structure design and optimization platform), RNASyn (CircRNA synthesis and modification platform), RNAPur (CircRNA purification platform), and RNAQua (CircRNA quality analysis and control). Relying on the complete technology platform, we can realize the efficient preparation and purification of circRNA, and provide the whole process and high quality CRO service for circRNA in vitro preparation for universities and research institutions.

A Great Layout
Yaohaibio’s New CRO Services for Novel Circular RNA Therapies!
Welcome to inquiry and discuss the businesses

Circular RNA Research-Grade Sample Preparation Service Platform

CircRNA purification platform
CircRNA quality analysis and control platform
CircRNA synthesis platform
CircRNA structure design and optimization platform
Cutting-edge "PIE" circularization technology; efficient intron and exon combination
Optimized design of CDS, IRES
Multiple purity testing solutions
Design and construction of circRNA template plasmids
CircRNA synthesis solution with the circularation rate >80%
Conventional experimental purification protocol
Individually developed chromatographic column purification process
Efficient circularization rate detection protocol

Services
Based on the gene sequences or amino acid sequences provided by customers, Yaohaibio provides a series of customized circRNA research services according to the actual requirements of customers. The business covers circRNA sequence structure design and optimization, in vitro transcription template construction, circRNA circularization and purification, identification of purity and circularization rate, etc., which can provide high-quality circRNA products and technical service for researchers.

Research-Grade Circular RNA in Vitro Preparation Services
Covering the entire life cycle of circRNA research-grade sample preparation and quality control

Structural design and template construction of circRNA
Synthesis and circularization of circRNA
Multiform purification of circRNA
Quality control of circRNA purity, circularization rate testing, etc.

Catalog of Prefabricated Circular RNA Products

Advantages of Technology Platform
High efficiency: Verified by HPLC, RT-PCR, etc., the circularization rate is > 80%
Strong process: Capable of providing 50-3000nt circRNA preparation service
Stability: Fluorescent protein expression can be detected one week after transfected cells, with strong stability
Flexibility: A mature chromatographic purification process developed independently and diversified purification methods are provided to meet the requirements of different experiment.
Rigor: Strict quality control methods and efficient circularization rate detection protocol
Advanced technologies: Senior technical team led by PhD, advanced experimental equipment and strict quality assurance team, quick response to meet customer delivery requirements
All-round: Provide one-stop service from sequence to finished product and provide circularization service for linear RNA
Customization: According to customer needs, RNA circularization services can be customized to achieve private customization

Cases
Cell evaluation: CircRNA prepared in vitro, eGFP is expressed efficiently and stably, and strong fluorescence signals can still be detected one week after transfected cells.