Yaohaibio’s Full Ecological Recombinant Expression System of Nanobody
Full-range customization services
Nanobody (Nb), namely VHH (variable domain of heavy chain of heavy-chain antibody), was first reported by Belgian scientists in the journal Nature in 1993. It is a camelaceae (camel, alpaca, alpaca and their close relative species), a single-domain antibody composed of a variable region (VHH) of a natural heavy chain antibody that lacks a light chain. The antibody contains only one heavy chain variable region (VHH) and two conventional CH2 and CH3 regions. The VHH crystal is a cylinder with a diameter of 2.5 nm and a length of 4 nm. The molecular weight is only 15 kDa, so it is also called Nanobody (Nb).
Traditional Antibody (IGG)
Heavy chain antibody (HCAbs)
Nanobody (Nbs)
Monovalent nanobody
Bivalent nanobody
Bispecific nanobody
Multivalent nanobody
Yaohabio has rich experience in nanobody R&D and production. With a mature technical platform, advanced production equipment, and pioneering technology in the full ecological expression system of nanobody, Yaohaibio is committed to providing customers with one-stop R&D, production and preparation services from strain construction, multifunctional nanobody expression and purification, and large-scale production, efficiently and flexibly meet customer requirements for different experiments or projects.
Full ecological expression system
E.coli prokaryotic expression system
Yeast eukaryotic expression system (Pichia pastoris)
Mammalian cell expression system
Diversified nanobodies
Single-domain nanobody
Divalent nanobody
Multivalent nanobody
Mature and complete purification platform
Complete purification platform
Combined purification method
Efficient and flexible
μg to kg
Expression of nanobody is up to 10g/L
Production scale 7-2000L
At present, Yaohabio has established a fully ecological recombinant expression system of nanobody. The existing expression systems include: E.coli prokaryotic expression system, yeast expression system (Pichia pastoris), mammalian cell expression system. We are proficient in using a variety of host bacteria to provide customers with high-quality nanobodies according to the actual requirements of customers.
E.coli prokaryotic expression system
Rich development experience of 20+ varieties
Flexible selection of different Escherichia coli hosts
Efficient selection of different expression carriers
Yeast expression system (Pichia pastoris)
Rich development experience of 10+ varieties
Flexible selection of different Pichia yeast hosts
PAOX1 methanol-induced expression system
PGAP constitutive expression system
Mammalian cell expression system
Rich development experience of 5+ varieties of nanobodies
Transient expression of nanobodies
Stable expression of nanobodies
Scientific research level
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One-stop service for the whole process of target sequence from gene synthesis to expression purification
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Complete nanobody purification system and quality analysis system
Industrial level
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Large-scale nanobody preparation services
Based on the nanobody gene sequences or amino acid sequences provided by customers and selected host cells for expression, Yaohaibio provides a full range of one-stop customized nanobody services from gene synthesis to nanobody expression and purification services, and to scale production.
Steps | Services | Cycle | Delivery |
Engineering bacteria construction of nanobody expression (Pichia yeast, E.coli, or mammalian cells can be selected) | 1. E.coli expression system (cytoplasmic or periplasmic spatial expression) 2. Pichia yeast expression system (the methanol-induced expression system PAOX1 or the component expression system PGAP) can be selected 3. Mammalian expression system (expression of nanobody through transient or stable transformant) | 1. 1-2 weeks for Escherichia coli (excluding gene synthesis cycles) 2. 2-3 weeks for Pichia pastoris 3. Mammalian: 1-2 weeks for transient and about 2 months for stable transfection | For purified nanobodies: purified antibody detection reports by SD S-PAGE, SE C-HPLC (optional), RP-HP LC (optional) and CE-SDS (optional) purity analysis methods |
Low expression purification (label) | Express the constructed nanobody of the engineered strain, and purify 1L of expression volume of protein (label recommended). | 2-3 weeks | |
Large-scale expression purification | Ferment nanobody samples in large scale, and express and purify the fermented nanobody at the same time. | 5-8 weeks |
Mature nanobody expression and purification platform, rich technical and project experience, and one-stop services.
Expert team
A strong technical team, rich biological technical background, and various expression and purification platforms led by Ph.D, can provide one-to-one special services.
Full-domain expression platform
There’s stable nanobody expression platforms such as prokaryotic cell E. coli, eukaryotic Pichia pastoris and mammalian cell CHO. Customers can flexibly select expression platforms according to their requirements to reduce trial and error costs.
Complete quality system
Various purity analysis methods including SDS-PAGE, SEC-HPLC, RP-HPLC and the CE-SDS based on capillary electrophoresis, and a deep experience reserve for nanobody quality evaluation system.
Efficient capture and purification system
With rich experience in nanobody purification, we have accumulated various nanobody purification methods such as affinity chromatography, ion chromatography, hydrophobic chromatography, etc., which can be flexibly combined with different purification protocols, and the purity can be up to 98%.
Large-scale production
Production service sizes from 7 to 2000 L are available.