Customized Preparation Service of Research-Grade mRNA Samples

The outbreak of the new crown epidemic in 2020 has brought mRNA technology to the forefront. mRNA vaccines and drugs have attracted worldwide attention, and related researches has become unprecedented. Nowadays , the number of mRNA R&D projects has been increasing exponentially, resulting in rapid research progress in various fields such as tumor immunity, protein replacement therapy, regenerative medicine, and cell therapy.

In February 2022, Yaohaibio launched the mature and complete service platform for the preparation of research-grade "RNASci" mRNA samples. There are four technical modules that provide customers with one-stop services, such as sequence design and optimization, gene synthesis, recombinant plasmid preparation, linearization template preparation, IVT and purification, and mRNA quality control. The services cover the entire life cycle of mRNA product from design to prototyping, fully empowering mRNA vaccine and drug development.

One-Stop Solution

Sequence Design and Optimization
Linearized Template Preparation
mRNA Quality Control and Analysis
Gene Synthesis
IVT 
Recombinant Plasmid Preparation
mRNA Purification

Service Advantages
01 Integrated service process
From front-end sequence design and optimization, gene synthesis to back-end mRNA synthesis and quality control analysis
02 High-quality structure design and optimization platform
Professional mRNA structure design and optimization to facilitate efficient mRNA expression
03 Mature purification platform
General & individually developed combined purification process to provide high-quality mRNA services
04 Prefabricated/customized mRNA
Flexible and diverse options to meet different experimental / project needs

Platform technical contents
Optimal design of 5'UTR and 3'UTR
Optimal design of CDS area
Optimal design of PolyA tail
Design and construction of mRNA template plasmid
Synthesis of conventional mRNA (cap structure, PolyA tail structure)
Synthesis of nucleotide-modified (eg ψ, N1ψ, 5mC) mRNA
mRNA service platform
mRNA structure design and optimization platform
mRNA synthesis and modification platform
mRNA purification platform
mRNA quality analysis and control platform
RNASci 
RNADes
RNASyn
LiCl precipitation purification
Magnetic bead purification
Individually developed chromatographic column process purification
Agarose gel electrophoresis, CE purity testing system
Translation expression testing system
Cap rate testing system (cooperative testing)
PolyA distributed testing system (cooperative testing)
RNAQua
RNAPur

Yaohaibio provides customers with not only the various prefabricated mRNA products, but also the customized synthesis services of research-grade mRNA. The services are continuously upgraded to fully meet the demands of different customers for different experiments or projects.

According to the different project requirements of customers for mRNA, a variety of purification methods can be provided, including the mature chromatographic purification process invididually developed by Yaohaibio to prepare high-quality, high-purity mRNA products. The prefabricated mRNA is well expressed in cells.

Oligo-dT purification is adopted to remove micromolecule process related impurities. The purity of the prefabricated products is more than 95%. The content of dsRNA tested by dsRNA detection kit (ELISA) in the prefabricated products is less than 0.006%.

The prefabricated mRNA prepared by Yaohaibio is expressed well in 293T transfection