The most important role of mRNA, also known as messenger RNA, is to directly direct protein synthesis through replication, transcription and translation, i.e. copying the genetic information carried by DNA in the nucleus, transcribing it into the cytoplasm, and translating it into proteins. Recent researches show that 90% of human diseases are protein-related and are associated with mRNA properties, therefore, the mRNA drugs and vaccines are of great significance and value.

As a leader in CDMO services for microbial expression systems in China, based on its strong R&D technology platform, mature quality management and comprehensive service capability, Yaohaibio has recently launched the mature and complete "RNASci" research-grade mRNA sample preparation service platform, which consists of 4 major technology modules: RNADes (mRNA structure design and optimization platform), RNASyn (mRNA synthesis and modification platform), RNAPu (rmRNA purification platform), RNAQua (mRNA quality analysis and control) , across the full life cycle of mRNA from design to formulation.

RNADes
mRNA  Structure Design and Optimization Platform

Platform technical features:
Optimial design of 5' UTR and 3' UTR; 
Optimal design of CDS area; 
Optimal design of PolyA tail 

Platform features:
Yaohaibio has established a natural UTR bank containing UTR sequences of common highly expressed proteins such as α-hemoglobin A1 (HBA1), with diversified UTR sources to match the appropriate UTR sequences for different species. 
Able to optimize the natural 5'UTR to help transcribe the template more efficiently; 
International cutting-edge design strategies for structures such as termination codons, PolyA tails, etc. 
Mature general codon optimization methods; special optimization needs can be done in cooperation with professional AI algorithm team.

RNASyn
mRNA Synthesis and Modification Platform

Platform technical features:
Design and construction of mRNA template plasmids; 
Synthesis of conventional mRNA (containing Cap and PolyA). 
Synthesis of nucleotide-modified mRNA (e.g., ψ, 5mC). 

Platform features:
Highly productive and stable capping process, which can achieve a capping efficiency over 95%. 
Integrated PolyA tail transcription and formation with more uniform distribution. 
Diversification of mRNA-modified nucleotides, which can effectively reduce the adverse immune response of mRNA in humans. 
Flexible plasmid template designs to meet specific customer needs

RNAPur
mRNA Purification Platform

Platform technical features:
LiCl precipitation purification; 
Oligo dT magnetic bead purification; 
Chromatographic column process purification. 

Platform features:
Diversified purification methods, a comprehensive purification solution consisting of tangential flow filtration and multiple chromatographic packing, which can effectively remove impurities from mRNA crude products and meet the application scenarios with high quality requirements; 
Mature and comprehensive solutions for LiCl precipitation, magnetic bead purification and chromatographic purification, especially that for the chromatographic purification solution, which is completely developed by Yaohaibio, can effectively remove various impurities generated during the preparation of mRNA.

RNAQua
mRNA Quality Analysis and Control Platform
Platform technical features:
Denatured gel electrophoresis (formaldehyde) purity testing system; 
Translation expression detection system 
Capping rate testing system (under construction). 
PolyA distribution detection system (under construction). 
Platform features:
Matching quality analysis and control systems for research-grade sample concentration and purity;
Able to meet special quality control requirements such as mRNA translation testing, capping rate, tail distribution, etc.

Research-Grade mRNA Product Services

Besides the customized synthesis of research-grade mRNA samples, Yaohaibio also provides various prepreparaed mRNA products to meet different experimental or project demands of different customers.

Preprepared mRNA Product Catalog
mRNA_eGFP (Transfection Control), 500 ng/ μL
mRNA_1273 (Moderna Vaccine), 500 ng/ μL
mRNA_162b2 (Pfizer Vaccine), 500 ng/ μL

Customized mRNA Services: 
Design and construction of mRNA template plasmids;
Preparation of classical mRNA (cap structure, PolyA tail structure);
Preparation of nucleotide modified mRNA (pseudouracil, N1 methyl pseudouracil, 5 methylcytidine);
Other customized mRNAs.

Customized mRNA Service Features:
·High quality: Professional mRNA structure design and optimization to facilitate mRNA expression efficiency.
·Flexibility: FreeCut template plasmid, which allows flexible selection of linearization method.
·Customization: A variety of nucleotide-modified mRNA can be prepared to support tailor-made customization and to meet the special customization requirements of customers.
·Diversity: A variety of purification methods can be provided, including self-developed mature chromatographic purification processes, which can meet the requirements of different experiments.
·Flexibility: The flexible customized scale can meet the different scale requirements from 50 μg to 10 mg.

With stability in progress, innovation never stops. mRNA is an important direction for the future development of biopharmaceuticals, and based on the strategic deployment of gene therapy development, Yaohaibio will comprehensively lay out the development of mRNA technology and platform to gradually build and improve the service ecology from research-grade mRNA sample preparation to GMP-grade one-stop mRNA service solutions; actively help break through the barriers of mRNA drug development and assist more pharmaceutical companies to accelerate the development, clinical trials and marketing of mRNA drugs, and benefit more patients as soon as possible.

As a witness of the booming development of domestic biomedicine industry, Yaohaibio insists on innovation and development, and is firmly on the road of continuously improving the R&D, production, quality management and control service system. We are devoted to providing the more efficient and quality one-stop CDMO services with the solid basis of platform technology.

Serve with heart and create the future together. With the mission of “establish global standards, promote new drugs development, and achieve a healthy life”, Yaohaibio will continue to facilitate the innovation of new drugs worldwide.

A Gift for Forwards&Likes

Good news: technological innovation! Yaohaibio’s one-stop solution for the customized synthesis of research-grade mRNA samples is officially launched, which actively helps break through the barriers of mRNA drug development and continuously facilitates the innovation of new drugs worldwide. To make orders, you can directly scan the following customer service WeChat and get exclusive services! Meanwhile, there is also a special plush toy for those who shared the article to the Moments and collected 28 Likes! Let’s forward it!